The ligand and receptor specificity of factor VIIa/TF-stimulated monocyte-derived macrophage ROS production after 24 hours of coincubation with LPS in serum-free media. Factor VIIa induced significant augmentation of ROS production compared with the absence of factor VIIa (control-no VIIa), but augmentation was not observed with native factor VII or active site inactivated factor VIIa. Augmentation of ROS production by factor VIIa was abolished in the presence of anti-TF antibody (VIIa + anti-TF Ab) but was unaffected by a control antibody (VIIa + control Ab). *P < .0001 compared with control.