Fig. 3.
Expression of bcl-2 protein in human CLL cells at 4, 24, and 48 hours after incubation with medium or 0.15 or 0.015 μmol/L of depsipeptide. The cells were obtained from CLL patients after informed consent was obtained, were isolated, and were cultured at 5 × 106/mL in medium or depsipeptide. Cell lysates were prepared and protein concentration was quantified using the BCA method (Pierce). Two micrograms of protein per lane from the CLL cell lysates was loaded onto a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and electrophoresed. Bcl-2 protein was detected using an anti–bcl-2 monoclonal antibody (Dako). Lane equivalent loading was verified by assessment with Fast Green staining (not shown).
