Fig. 5.
Expression of p27 protein in human CLL cells at 4, 24, and 48 hours after incubation with medium or 0.15 and 0.015 μmol/L of depsipeptide. The cells were obtained from CLL patients after informed consent was obtained, were isolated, and were cultured at 5 × 106/mL in medium or depsipeptide. Cell lysates were prepared and protein concentration was quantified using the BCA method (Pierce). Five micrograms of protein per lane from the CLL cell lysates was loaded on a 10% SDS-PAGE gel and electrophoresed. The p27 protein was detected using an anti-p27 polyclonal antibody (Oncogene). Lane equivalent loading was verified by assessment with Fast Green staining (not shown).
