Fig. 1.
The expression of ETA and ETBreceptors on CNS-EC and HUVECs. RNA from cells from 3 different CNS-EC cultures and 1 sample of HUVECs was extracted and examined using PCR primers for ETA (lanes 1 through 4), ETB (lanes 5 through 8) receptors, and β-actin (lanes 9 through 12). The CNS-EC are grouped in lanes 1, 5, and 9; 2, 6, and 10; and 3, 7, and 11. HUVEC RNA is shown in lanes 4, 8, and 12. M designates the marker lane.