Organ sequestration of radiolabeled RRBC after in vivo Ad-mediated FcγRIIA cDNA gene transfer. Sprague-Dawley rats were administered AdFcγRIIA or AdNull intravenously (109 pfu). After 48 hours, 51Cr-labeled opsonized (IgG-coated) RRBC or nonopsonized RRBC as controls were injected intravenously. After 120 minutes, the liver, lung, and spleen were removed and homogenized. Total radioactivity of the organs, measured in a gamma scintillation counter, is presented as a percentage of the total dose of injected radioactivity (dpm). (A) Organ sequestration after administration of nonopsonized RRBC. (B) Organ sequestration after administration of opsonized RRBC. Shown are mean percentages with standard errors from 4 animals in the control group, 6 animals in the AdNull-infected group, and 4 animals in the AdFcγRIIA-infected group.
