Immunostaining of HFE. HtTA-HFE cells were grown on coverslips for 4 days in the absence (A,C) or presence (B,D) of 1 μg/mL doxycycline. Immunostaining was perfromed as described in the methods. Permeabilzed cells were incubated with the antiserum directed against the C-terminal peptide (A,B), unpermeabilized cells were incubated with the antiserum directed against the N-terminal peptide (C,D).