Fig. 5.
Autologous antileukemia T-cell lines adhere to autologous bone marrow stroma. Results indicate the mean ± SD number of cells bound per surface area from 1 representative experiment (3 independent experiments with T-cell lines from 6 patients). Background adherence was determined by the binding of these cells to BSA-coated wells. Stromal cell layers were established in 96-well plates as described in Materials and Methods. The blocking antibodies anti-CD29, anti-CD49d/VLA-4, and anti-CD106/VCAM-1 were used at 5 μg/mL.