Inhibition of SIRP1ex binding by CD47-specific MoAbs CC2C6 and BRIC126. (A) CCRF-CEM cells were preincubated with CD47-specific MoAbs CC2C6, and BRIC126 (filled histogram), or a nonbinding control antibody (black line) and then labeled with biotinylated SIRP1ex and stained with SA-PE. Inhibition of SIRP1ex binding to CCRF-CEM was analyzed by flow cytometry (n = 3). (B) CCRF-CEM cells were preincubated with CD47-specific MoAbs CC2C6, BRIC126, 2D3, or a nonbinding control antibody. Adhesion of pretreated CCRF-CEM cells to immobilized SIRP1ex (2 μL of 20 μg/mL solution) was analyzed by microscopy (original magnification × 40) (n = 3).