Junctional nucleotide and amino acid TCRBV1, TCRBV13S1, and TCRBV15 sequences obtained from PBMC and thymocytes from the OS patient and from the age-matched control (C). Amino acid sequences are shown on the right side and displayed using the standard 1-letter code. Only the last amino acids of the TCRBV chains and the first 5′ amino acids of TCRBJ segments are shown. N/P, nontemplated and palindromic nucleotides; freq, number of each clone in the total cDNA clones characterized; °, junctions in which D regions cannot be assigned; *, clone with the following N nucleotides: CTGTCTCCCCGCATGTTAGCTCCTC.