Junctional nucleotide and amino acid TCRDV3 sequences obtained from PBMC and thymocytes from the OS patient and the control (C). Germline sequences are indicated at the top and are double underlined. Amino acid sequences are shown on the right side and displayed using the standard 1-letter code. Only the last amino acids of the TCRDV chains and the first 5′ amino acids of TCRBJ segments are shown. N/P, nontemplated and palindrome nucleotides; freq, number of each clone in the total cDNA clones characterized.