Expression of GPV in Dami cells. (A) Dami cells were analyzed by flow cytometry using MoAbs against GPV (V.1) or the GPIIb-IIIa complex (ALMA.17) and FITC-conjugated goat antimouse IgG. Histograms are representative of 5,000 cells. Neg corresponds to isotype-matched negative control. (B) RT-PCR analyses were performed on total RNA from HL60, K562, HEL, and Dami cells. The GPV PCR products were identified by 2% agarose gel electrophoresis and ethidium bromide staining and neg corresponds to RT-PCR without reverse transcriptase.