Sequence of the GPV gene promoter and DNA fragments of the 5′-flanking sequence used for DNase I protection assays. The GPV promoter was numbered from an arbitrary start site common to Dami cells and platelets, which conforms to the consensus start of platelet TATA-less gene (Table 2). (A) Alignment of the fragments I to VII with the −1432/+21 GPV 5′-flanking segment. The fragments were checked by sequencing before 5′-end-labeling and use in DNase I protection assays. Footprinting analyses of III, IV, VI, and VII are reported in Figs 4 through 7. (B) Sequence of the GPV promoter. The transcription start site is denoted +1, the intron sequence is in lowercase characters, and the intron donor splice site is in bold characters.