Analysis of GPV gene reporter/Exonuclease III deletion constructs after transient transfection in Dami and HeLa cells. (A) Schematic representation of the −1413 to +25 5′-flanking region of the human GPV gene linked to the luciferase reporter gene (luc) in the pGL3 vector. Binding sites for transcription factors, functionally identified by DNase I footprinting or detected by sequence analysis in the case of R_IIb-817, are indicated by the symbols: (▪) GATA, () Ets, (•) Sp1 family, (▴) STAT, and (⧫) sites homologous to the GPIIb repressor. (B) On the left is a schematic diagram of the different constructs: (upper) a control SV40 promoter construct (pGL3/SV40) used to set the 100% standard activity; (middle) progressive 5′ to 3′ deletions of the −1413/+25 construct; and (lower) an inverted construct (pGL3/−1413R) of the −1413/+25 segment. On the right, the luciferase activities of the constructs transfected into Dami (shaded histograms) or HeLa cells (open histograms) are given as percentages of the control (pGL3/SV40) activity. Values were corrected for transfection efficiency by cotransfection with a sea pansy/luciferase construct under the control of the thymidine kinase promoter. Points are the mean ± SEM of at least 3 experiments performed in triplicate.