Reporter deletion studies of the GPV −103/+25 region. On the left is a schematic diagram of the GPV promoter/luciferase constructs. Sequential deletions of pGL3/−103 progressively removed the GATA-71/Ets-66 and Ets-42 domains and the luciferase activity was tested after transfection into Dami and HeLa cells. The pGL3/−103 construct containing the 103-bp flanking region upstream of the firefly luciferase gene is the same as in Fig 8B and had 64% activity as compared with pGL3/SV40. The lower construct (pGL3/SV40-103), which corresponds to the 103-bp flanking region of GPV linked to pGL3/SV40, was used to search for enhancer activity. On the right, the luciferase activities of the different constructs transfected into Dami and HeLa cells are given as percentages of the control (pGL3/SV40) activity. Cotransfection with a sea pansy/luciferase construct was used to normalize for transfection efficiency, and points are each the mean ± SEM of at least 3 experiments performed in triplicate.