Reporter mutation analysis of the GPV GATA-71/Ets-66 tandem. On the left is a schematic diagram of the GPV promoter/luciferase constructs. Crossed-out GATA-71 or Ets-66 corresponds to mutation of these sites and is noted as “mut” (see Materials and Methods). “▵” corresponds to deletion of the GATA-71 site. On the right are the luciferase activities of the constructs after transfection into Dami and HeLa cells. Cotransfection with a sea pansy/luciferase construct was used to normalize for transfection efficiency. Each point is the mean ± SEM of at least 3 experiments performed in triplicate.