Fig. 2.
Induction of apoptosis in Jurkat and JPX-9 cells by PDTC determined by flow cytometry. Jurkat and JPX-9 cells were preincubated with 30 μmol/L of CdCl2 for indicated time intervals, washed with PBS, and further cultured with 50 μmol/L of PDTC for 24 hours. After cultivation, apoptotic cell death was determined by flow cytometry as described in the text. Numbers in parentheses are percentages of cells with hypodiploid DNA. The results are representative examples of 6 similar experiments.