Fig. 3.
Expression of pro-caspase-3 in Jurkat and JPX-9 cells determined by Western blotting. Jurkat and JPX-9 cells were preincubated with or without CdCl2 for 6 hours. After washing with PBS, the cells were further cultured with or without 50 μmol/L of PDTC for 24 hours. After cultivation, we examined the expression of pro-caspase-3 as described in the text. Results are representative examples of 6 similar experiments. Note that the expression of pro-caspase-3 in Jurkat cells is significantly decreased by PDTC irrespective of CdCl2 pretreatment, whereas that of JPX-9 cells in the presence of PDTC is almost suppressed by preincubation of the cells with CdCl2.
