Fig. 5.
Enzyme activity assay of caspase-3 in JPX-9 cells. JPX-9 cells were preincubated with or without CdCl2 for 6 hours, and further cultured in the presence or absence of 50 μmol/L of PDTC for 24 hours. After incubation, the enzyme activity of caspase-3 was determined as described in the text. Results are representative examples of 5 similar experiments. Note that caspase-3 enzyme activity is markedly increased in CdCl2-untreated JPX-9 cells by PDTC, whereas it is significantly suppressed by preincubation of cells with CdCl2.
