Bcl-2 expression in Jurkat and JPX-9 cells. (A) Jurkat and JPX-9 cells were preincubated with or without CdCl₂ for 6 hours, washed with PBS, and further incubated with or without PDTC (50 μmol/L) for 24 hours. After incubation, the expression of Bcl-2 was examined as described in the text. (B) JPX-9 cells were preincubated with or without CdCl₂ for 6 hours, washed with PBS, and further incubated with or without NAC (50 mmol/L) or LLL-CHO (10 μmol/L) for 24 hours. After incubation, the expression of Bcl-2 was examined as described in the text. Note that the expression of Bcl-2 in Jurkat or JPX-9 was not different in each culture condition. Results are representative examples of 5 similar experiments.