Iron loading in Hx −/− tissues. Liver (A,B), spleen (C,D) and kidney (E,F) sections from Hx −/− (A,C,E) and wild-type (B,D,F) mice stained with Prussian blue for detection of ferric iron. Liver and spleen show iron deposits in Kupffer cells and macrophages, respectively, without differences between knockouts and controls. Kidney of Hx −/− has much more iron loading compared with controls: intense blue staining is evident in proximal cortical tubules. Bar, 20 μm.