Analysis of renal damage after injection of 0.2 mg/g of phenylhydrazione. (A) Measurement of iron loading in the kidney of Hx −/− and wild-type mice 7 days after phenylhydrazine treatment. Mean number ± SD of Perls’ positive granules per tubular cell. At least 200 cells for each animal were counted. A total of 6 mice for each genotype was used (P < .001). (B) Lipid peroxidation estimated as MDA levels of tissue homogenates of Hx −/− and wild-type mice 7 days after phenylhydrazine treatment. Data represent mean ± SEM from 5 mice for each genotype (P < .01). Kidney from Hx −/− mice, which consistently shows iron loading, has significantly greater oxidative damage than that from control mice.