Subunit polypeptides of purified fibrinogen Niigata examined by SDS-PAGE. To each lane 1.5 μg of protein was loaded on 7.5% to 12.5% gradient gel under the reducing (lanes 1 and 2) or nonreducing (lanes 3 and 4) conditions and the proteins were stained with Coomassie Brilliant Blue R250. Lanes 1 and 3 are normal fibrinogen and lanes 2 and 4 are patient’s fibrinogen.