Fig. 7.
Cross-linking of CD16 but not CD38 induces an increase in intracellular Ca2+. Purified, IL-2–activated NK cells were loaded with Fluo-3, warmed to 37°C, and, at the time indicated by the left arrow, treated with anti-CD38 (IB4) F(ab′)2 (•) anti–MHC-1 (W6/32) MoAb (▿), or anti-CD16 (3G8) MoAb (▪). At the time indicated by the right arrow, antibodies were further cross-linked by the addition of rabbit F(ab′)2 antimouse IgG. Cytolysis mediated by PBLs from this donor is shown in Fig 1A. This experiment has been repeated 4 times, with similar results.
