(A) RT-PCR analysis for murine SDF-1β mRNA. Using cDNA from M2-10B4 cells (lane 3) and plasmid DNA encoding murine SDF-1β as a positive control (lane 2), PCR fragments of the expected size of 296 bp were amplified in both test samples (100-bp marker in lane 1). (B) Chemotaxis of the Reh B-cell line in response to conditioned medium (CM) from M2-10B4 cells. Compared with medium (Control), M2-10B4 CM at different concentrations (100, 50, and 20 vol%) induced chemotaxis of Reh cells. This migration was inhibited by preincubation of Reh cells with 30 μg/mL CXCR4 MoAb. Error bars indicate the range of duplicate samples.