Detection of HHV-6 DNA by PCR with 7 distinct primer pairs that target different regions of the HHV-6 genome. The PCR products were subjected to electrophoresis and stained with ethidium bromide. The products were all of the predicted sizes. (A) HHV-6 U4 gene. (B) U31 gene. (C) U57 gene. (D) U67 gene. (E) U89 gene. (F) U94 gene. (G) HHV-6 sequence located between U91 and U92 genes. Lane 1, patient; lane 2, patient’s husband; lane 3, their daughter; lane 4, healthy adult donor as a negative control; lane 5, HHV-6B–infected cord blood cells as a positive control; lane M, ◊X174/HincII-cut DNA size marker.