Thymic repopulation by cultured CD34⁺CD38⁻Lin⁻ cells in vitro. (A) Flow cytometric analysis of the progeny of CD34⁺CD38⁻Lin⁻ cells after pre-FTOC culture for 10, 24, and 38 days in TPO + FL + SCF. Dot plots show CD38 FITC versus CD34 PE staining. (B) Flow cytometric analysis of thymocytes recovered from FTOC initiated with cells shown above in (A). Dot plots show CD4 APC versus CD8β PE, anti-TCR–γδ FITC versus CD3 APC and CD56 PE versus CD3 APC stainings. The latter staining was not performed on thymocytes from FTOC initiated with CD34⁺CD38⁻Lin⁻ cells cultured for 10 days. Quadrants were set to include 99% of cells stained with isotypic control antibody in lower left quadrants. Data shown are representative of 2 independent experiments with 2 different donors.