American Society of Hematology

Fig. 1.

$Fig. 1. Timed expression of λ5, B220, and CD19 on Lin−/loSca1+c-kit+ cells cultured in FL + IL-7. Cultures were initiated with Lin−/loSca1+c-kit+ (all hCD25−) cells isolated from transgenic mice expressing hCD25 under control of the λ5-promoter.35 Cells were supplemented with FL + IL-7, and at each indicated time point, cells were analyzed for the coexpression of B220, hCD25(λ5), and CD19 by flow cytometry. One representative experiment of 3 individual experiments is shown. In initial control experiments in which hCD25− and hCD25+ cells were sorted from FL + IL-7–stimulated cultures, endogenous λ5 mRNA was detected by RT-PCR in the hCD25+ fraction, but not in the hCD25− fraction, demonstrating the specificity and sensitivity of hCD25 expression as a marker for λ5 expression (data not shown).$

Timed expression of λ5, B220, and CD19 on Lin^−/loSca1⁺c-kit⁺ cells cultured in FL + IL-7. Cultures were initiated with Lin^−/loSca1⁺c-kit⁺ (all hCD25⁻) cells isolated from transgenic mice expressing hCD25 under control of the λ5-promoter.35 Cells were supplemented with FL + IL-7, and at each indicated time point, cells were analyzed for the coexpression of B220, hCD25(λ5), and CD19 by flow cytometry. One representative experiment of 3 individual experiments is shown. In initial control experiments in which hCD25⁻ and hCD25⁺ cells were sorted from FL + IL-7–stimulated cultures, endogenous λ5 mRNA was detected by RT-PCR in the hCD25⁺ fraction, but not in the hCD25⁻ fraction, demonstrating the specificity and sensitivity of hCD25 expression as a marker for λ5 expression (data not shown).

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