In vitro myeloid and lymphoid potential of progeny derived from FL + IL-7–stimulated Lin^−/loSca1⁺c-kit⁺ cells. A total of 500 Lin^−/loSca1⁺c-kit⁺ cells/mL was cultured in the presence of FL + IL-7 for 12 days, at which time cells were washed and either transferred to myeloid MC cultures (duplicates) supplemented with G-CSF + GM-CSF + IL-3 + KL (A) or plated at a density of 1 cell per well in serum-depleted medium supplemented with FL + IL-7 (B). The CFU-GM content was analyzed 7 to 10 days after transfer and the proB-cell potential was evaluated (as described in Fig 3) after 12 to 20 days of culture. The results represent the mean (+SEM) of 3 individual experiments.