Dose response of HGF on secretion of IL-11 from osteoblasts. (A) Saos-2 cells, (B) HOS cells, and (C) hOB cells were incubated with 0 to 100 ng/mL HGF for 48 hours and IL-11 in conditioned medium was measured in duplicates by ELISA. Cell line experiments were repeated at least 3 times, and similar results were obtained with hOB cells from 4 different donors. Data are means ± SD from 1 representative experiment of 3.