c-Maf–expressing cells downregulate Bcl-2 protein levels. (A) Cells expressing MEnT have decreased Bcl-2 protein levels. Western blot analysis of MEnT and Bcl-2 protein expression in uninduced or induced HL-60 clones (clones 1 and 2) containing the ZnSO₄ inducible MEnT Myb dominant-interfering construct. (B) Bcl-2 protein levels decrease coordinately with c-Maf expression. Western blot analysis of c-Maf, Bcl-2, and actin control protein levels in HL-60 c-Maf–containing clone 6 incubated with ZnSO₄ for the indicated time periods. Vector control cells were treated identically and assayed for Bcl-2 and actin protein levels (CB6 control).