Fig. 1.
LTB4 synthesis in PBM from healthy controls and AIDS subjects. PBM were isolated as described in Materials and Methods and plated at 1 × 106/mL in Dulbecco’s modified Eagle’s medium (DMEM). Maximal LTB4 release was determined after stimulation with A23187 (10 μmol/L) and AA (50 μmol/L) for 30 minutes at 37°C. Medium was analyzed for LTB4 by EIA and expressed as pg/106 cells, n = 10, *P < .001. Healthy control PBM LTB4 levels were 4,797.2 ± 884.5 pg/106 cells.
