5-LO and FLAP expression in PBM from control and AIDS subjects. Equal amounts (20 μg of protein) of crude cellular lysate from PBM were subjected to immunoblot analysis for 5-LO and FLAP as described in Materials and Methods. Top panel, representative autoradiograph of a Western blot demonstrating the amount of 5-LO (left) and FLAP (right) in PBM from healthy control and HIV-infected subjects. Lower panel, relative expression of 5-LO and FLAP in PBM from healthy control and HIV-infected subjects, as assessed by densitometry and expressed as a percent of values derived from cells from healthy control subjects. Data represent the mean ± SEM from n = 8 subjects. *P < .001.