Effect of in vivo GM-CSF therapy on PBM LT synthesis in AIDS subjects. PBM were isolated on day 1, day 4, and day 8 of the study protocol as described in Materials and Methods and plated at 1 × 10⁶/mL in DMEM. Maximal LTB₄release was determined after stimulation with A23187 (10 μmol/L) and exogenous AA (50 μmol/L) for 30 minutes at 37°C. Medium was analyzed for LTB₄ by EIA and the value obtained for HIV-infected subjects was expressed as a percent of the LTB₄ values observed for AIDS subjects on day 1 (2,851.9 ± 448.7 pg/10⁶ cells). *P = .05, n = 8.