Fig. 5.
![Fig. 5. [3H]Eicosanoid profile of stimulated PBM from AIDS subjects before and during therapy with GM-CSF. Cells prelabeled overnight were incubated with 1 μmol/L A23187 for 30 minutes. HPLC elution profiles are displayed for [3H]AA metabolites synthesized by A23187-stimulated PBM from a representative HIV-infected subject on day 1 (A) and on day 4 (B). Peaks were identified by coelution with authentic standards, and the products were expressed as a percentage of incorporated radioactivity. This HPLC profile is representative of 6 subjects whose cells were radiolabeled.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/11/10.1182_blood.v94.11.3897/4/blod42312005x.jpeg?Expires=1735565362&Signature=D8MvnnXY4uRY1oC702v10l9p7CFEaXQQJNcjMjnBfwKANUVD34Oy56ULU9GODmC1i4n-Ysxr0rdxS1U~vtvxF9mVHU-Php7c2a0robF5f2yx67Y4O6wTd9uyjZTHHXxwZb1qQSAGzKw~qQjSuVdH9eW~ZSd849HF9SPSZSWMSeiwASGlDG0D7YPyNM0DRjgk5cD3C-sSaeMdSZIb-exILZO4pNngvC8S5NRe~u443kIQmMe0Qe1lQG6iDgmuWo24Rob-OUrau2fm~KExDgv83y30gICsYCtM1XuPucequwJ2fbEyNVtMrKR2-f5VVXOVTub843~CdpzLaFhIzfDU-g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
[3H]Eicosanoid profile of stimulated PBM from AIDS subjects before and during therapy with GM-CSF. Cells prelabeled overnight were incubated with 1 μmol/L A23187 for 30 minutes. HPLC elution profiles are displayed for [3H]AA metabolites synthesized by A23187-stimulated PBM from a representative HIV-infected subject on day 1 (A) and on day 4 (B). Peaks were identified by coelution with authentic standards, and the products were expressed as a percentage of incorporated radioactivity. This HPLC profile is representative of 6 subjects whose cells were radiolabeled.
