Fate of rpvWF after injection of a single bolus (35 RCoF IU/kg) into a vWF-deficient dog (see Fig 5): (Top) SDS-PAGE under reducing conditions/Western blot. (Bottom) vWF multimer pattern over time. Plasma samples taken before and at specified intervals after injection were analyzed electrophoretically on a 3% SDS agarose gel. Multimers were visualized using an anti-human polyclonal vWF antibody that cross-reacts with vWF from other species. Control lanes: recombinant vWF preparation before injection (rpvWF), normal dog plasma, normal human plasma. D_I-III, T_I-V: variants of homo- and hetero-dimers and tetramers (D, dimer; T, tetramer) as illustrated in Fig 4. Arrows indicate satellite band formation. M_r, apparent relative molecular mass.