Gene expression in individual CD34⁺c-kit⁺ AGM cells. CD34⁺c-kit⁺ cells from AGM were individually sorted into 0.2-mL microtubes containing lysis buffer. Multiplex single-cell RT-PCR analysis was performed. The gel shows analysis of CD34, c-kit, EPO-R, G-CSF-R, and Lmo2. In each case, position 1 does not contain any cells and serves as a negative control, whereas position 12 contains 10 cells. The remaining positions (2-11) represent the amplification reactions of single cells. The expected migration position and molecular weight of appropriate RT-PCR products is indicated.