Long-term reconstitution of lethally irradiated mice. (A) Percent survival of lethally irradiated wild-type mice transplanted with 2 different amounts of bone marrow cells from ICSBP−/− or ICSBP+/+ mice as indicated. (B) Induction of granulocytosis in wild-type mice transplanted with ICSBP−/− bone marrow cells. The graph shows the percentage of circulating neutrophils in peripheral blood from reconstituted mice. The results shown represent mean ± SEM of wild-type mice transplanted with 5 × 106ICSBP−/− bone marrow cells (dashed line; n = 14) or with 5 × 106 ICSBP+/+ bone marrow cells (solid line; n = 9). Total leukocyte number in peripheral blood of wild-type recipient mice at 8 weeks posttransplant was 20.9 × 106/mL (ICSBP+/+) and 21.4 × 106/mL (ICSBP−/−), respectively. (C) Peripheral leukemia in ICSBP−/− mice is rescued by lethal irradiation and subsequent reconstitution with wild-type bone marrow cells. The graph shows the percentage of circulating neutrophils in peripheral blood from reconstituted mice. The results represent mean ± SEM of lethally irradiated ICSBP−/− mice (dashed line; n = 16) or wild-type mice (solid line; n = 9) transplanted with 5 × 106 wild-type bone marrow.
