Effects of hIL-3 and hGM-CSF on survival of FDCP-mix cell transfects. (A) Cell viability was assessed either in the absence of cytokines (▪) or in the presence of 100 ng/mL hGM-CSF (□) or 100 ng/mL hIL-3 (). Results are expressed as percentage of control (10 ng/mL mIL-3) and are the mean values from at least 2 experiments ± SEM. (B) Dose-response of FDCP-mix cells expressing (i) hIL-3 R (•) or hIL-3 R/β_c (○) to hIL-3 (ii) hGM R (▴) or hGM R/β_c (▵) to hGM-CSF. Cell viability was assessed in the presence of hIL-3 or hGM-CSF (0 to 100 ng/mL) by trypan blue exclusion after 48 hours culture. Results are expressed as percentage of control (10 ng/mL mIL-3) and are the mean values from at least 3 experiments ± SEM. Similar results were obtained with at least 4 clones of each transfect.