Fig. 3.
Effects of hIL-3 and hGM-CSF on proliferation of FDCP-mix cells coexpressing hIL-3 R,βc or hGM R,βc. Proliferation was assessed by determining [3H] thymidine incorporation after culturing for 16 hours at 37°C. FDCP-mix cells expressing (A) hIL-3 R /βcand (B) hGM R /βc were stimulated by hIL-3 (0.01 to 100 ng/mL) and hGM-CSF (0.1 to 100 ng/mL), respectively. Results are expressed as percentage of control (10 ng/mL mIL-3) and are the mean values from at least 3 experiments ± SEM. Similar results were obtained with at least 4 clones of each transfect.