(A) Morphology of hIL-3 R,βc cells and hGM R,βc cells in culture. Cells expressing hIL-3 R,βc were cultured in (i) hIL-3 (10 ng/mL) alone or (ii) hIL-3 (10 ng/mL) in combination with murine cytokines that promote granulocyte-macrophage differentiation. Cells expressing hGM R,βc were cultured in (iv) hGM-CSF (10 ng/mL) alone or (v) hGM-CSF (10 ng/mL) in combination with granulocyte-macrophage differentiation conditions. Panels (iii) hIL-3 R,βccells and (vi) hGM-CSF R,βc cells show the morphology of cells cultured in granulocyte-macrophage differentiation conditions for comparison. Cytospin samples of cells were prepared after 7 days in culture and the morphology examined after May-Grunwald-Giemsa staining. Bar, 10 μm. Results are from an experiment representative of 3. Similar results were obtained with at least 3 clones of each transfect. (B) Dose-response of hIL-3 and hGM-CSF effects on morphology of hIL-3 R,βc cells and hGM R,βc cells respectively in culture. Cells expressing (i) hIL-3 R,βc or (ii) hGM R,βc were cultured in hIL-3 or hGM-CSF (0.1 to 100 ng/mL), respectively. Cytospin samples of cells were prepared after 7 days in culture and the morphology examined after May-Grünwald-Giemsa staining. Results are expressed as cell morphology (percentage of total cells scored). Cells were scored as blast, early granulocyte (EG), late granulocyte (LG), or macrophage (m/phage). Results are from a single experiment representative of 3. Similar results were obtained with at least 3 clones of each transfect.
