Fig. 5.
IFN-γ augments B7-2 gene transcription. MM6 cells (5 × 107 cells/point) were treated with medium alone or with 500 U/mL of IFN-γ. Nuclei were isolated at the indicated time points, and the rate of transcription of the B7-2 gene was then assessed by nuclear run-on analysis as described in Materials and Methods. Data presented are from 1 of 2 similar experiments. The graph was generated as described in Materials and Methods, with the relative values obtained after normalization of the bands’ intensities to the respective amounts of β-actin.
