Treatment of MM6 cells with IFN-γ increases B7-2 mRNA stability. MM6 cells were incubated for 18 hours in medium alone or in medium supplemented with 500 U/mL of IFN-γ. After 18 hours, cells were treated with 5 μg/mL of Act-D, and their total cellular RNA was collected and analyzed by Northern blot for B7-2 mRNA expression at the indicated time points. Data shown are from 1 representative experiment of 2 performed. The graph was generated as described in Materials and Methods, and data are presented as the relative amounts of B7-2 mRNA remaining after adding Act-D and normalizing to the respective amounts of GAPDH.