IFN-γ treatment of human peripheral blood monocytes augments B7-2 mRNA expression. Human peripheral blood monocytes were cultured for 3 hours in medium alone or in the presence of either 500 U/mL of IFN-γ or 10 ng/mL of LPS. Total cellular RNA was extracted and analyzed by Northern blot for B7-2 mRNA expression. The same membrane was rehybridized with GAPDH to control that equal amounts of RNA were loaded in each lane. Data shown are from 1 representative experiment of 2 performed.