FANCC and FAZF colocalize in nuclear bodies. 293 EBNA cells were transfected with pFlag-FAZF, pHA-FANCC, or pHA-L554P, or the pFlag parental vector (control panel), as indicated. The fixed and permeabilized cells were stained with antibodies specific for the epitope tags, followed by staining with fluorescent-conjugated secondary antibodies (Red = FAZF and Green = FANCC or L554P). Immunofluoresence was examined by confocal laser microscopy. For double labeling, the green and red channels were recorded independently and then overlaid. (A) Subcellular localization of FAZF. Original magnification ×63. (B) Colocalization of FANCC and FAZF in nuclear foci. Original magnification ×60. (C) The mutant L554P protein does not colocalize with FAZF.