American Society of Hematology

Fig. 6.

$Fig. 6. Time course of LPS-induced degradation of IκB and nuclear import of NF-κB. (A) Immunoblotting of IκB in nonstimulated (time 0) and TNF-–stimulated or LPS-stimulated THP-1 cells. (B) Electrophoretic mobility shift assay of NF-κB in nuclear fractions isolated from nonstimulated (time 0) and TNF-–stimulated or LPS-stimulated THP-1 cells. LPS from E coli 0127:B8 was used in concentration of 1 μg/mL and TNF- at 100 U/mL.$

Time course of LPS-induced degradation of IκB and nuclear import of NF-κB. (A) Immunoblotting of IκB in nonstimulated (time 0) and TNF-–stimulated or LPS-stimulated THP-1 cells. (B) Electrophoretic mobility shift assay of NF-κB in nuclear fractions isolated from nonstimulated (time 0) and TNF-–stimulated or LPS-stimulated THP-1 cells. LPS from E coli 0127:B8 was used in concentration of 1 μg/mL and TNF- at 100 U/mL.

This Feature Is Available To Subscribers Only