Fig. 3.
Fig. 3. Cotransfection of FcɛRIγ with CD4/PIR-A3 enhances cell surface expression in 293T cells. Fifty nanograms of pcDNA3 (□), CD4/PIR-A3 (▩), or CD4/PIR-A3R632L (▪) cDNA were transiently transfected in the absence or presence of 500 ng murine FcɛRIγ cDNA. Twenty-four hours later, the cultures were labeled with PE-conjugated anti-human CD4 for flow cytometric analysis.

Cotransfection of FcɛRIγ with CD4/PIR-A3 enhances cell surface expression in 293T cells. Fifty nanograms of pcDNA3 (□), CD4/PIR-A3 (▩), or CD4/PIR-A3R632L (▪) cDNA were transiently transfected in the absence or presence of 500 ng murine FcɛRIγ cDNA. Twenty-four hours later, the cultures were labeled with PE-conjugated anti-human CD4 for flow cytometric analysis.

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