Fig. 6.
Fig. 6. CD4/PIR-A3 induces NO production in ANA-1 macrophages. ANA-1 clones (1 × 106 cells/well) were stimulated with medium, 100 U/mL IFN-γ, 1 μg/mL goat antimouse IgG, 10 ng/mL LPS, and 40 μL anti-CD4, as indicated for 24 hours, as described in Materials and Methods. Cell-free culture supernatants were then assayed for NO production. (□), pcDNA3; (▩), CD4/PIR-A3; (▪), CD4/PIR-A3R632L.

CD4/PIR-A3 induces NO production in ANA-1 macrophages. ANA-1 clones (1 × 106 cells/well) were stimulated with medium, 100 U/mL IFN-γ, 1 μg/mL goat antimouse IgG, 10 ng/mL LPS, and 40 μL anti-CD4, as indicated for 24 hours, as described in Materials and Methods. Cell-free culture supernatants were then assayed for NO production. (□), pcDNA3; (▩), CD4/PIR-A3; (▪), CD4/PIR-A3R632L.

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