BSAP/Pax5A-expressing EML cells successfully acquire the myeloid lineage commitment marker CD11b. (A) B220⁺/CD11b⁻ EML cells were placed in culture at 3 × 10⁵ cells/mL and then induced to differentiate into B220^hi/CD11b⁺ and B220^lo/CD11b⁺ cells with 10% WEHI-3B conditioned supernatant (contains murine IL-3) and 10 μmol/L ATRA. Flow cytometry was performed on days 0 and 3. Control EML clones EML/Hyg-1 and EML/Hyg-3 and Pax5A/BSAP-expressing EML clones EML/Pax5A-3 and EML/Pax5A-5 were induced to differentiate as described in (A). On days 1, 2, and 3 of differentiation, a small sample of cells was removed from the culture, and differentiation to B220^hi/CD11b⁺ (B) and B220^lo/CD11b⁺ (C) cells was determined by flow cytometry.