Fig. 6.
![Fig. 6. PP1 dose-dependently inhibits alboaggregin-A–induced tyrosine phosphorylation of FcR γ-chain, Syk, and PLCγ2.Platelets were preincubated for 3 minutes at 37°C with 0.25% dimethyl sulfoxide (DMSO) or various concentrations of PP1 before stimulation with alboaggregin-A (3.5 μg/mL) for 1 minute. Platelet suspensions were then lysed and either Syk or PLCγ2 immunoprecipitated. Precipitated proteins were then separated by SDS-PAGE and immunoblotted with 4G10 [A(i) and B(i)] and subsequently with either anti-Syk [A(ii)] or anti-PLCγ2 [B(ii)]. Immunoblots shown are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/5/10.1182_blood.v94.5.1648/5/blod41731006w.jpeg?Expires=1735842134&Signature=r8VwfhJ~k~FOvByxPRYS~K3z7Z~ttHR935BjF3QWHywZZ90nK6HkWUwc~jpKyPL-Yscm4eL7stIJybeAihIcWgYVtd5XNFZ0REG94aNxODF8jX7Gzmj~yEF7bpc578EoMh~eAvScUj5Gjcn5rvyiN-7eIEEDwo37Ef7MiPW6Oj6rIoAQD0o8mZghOMSR0WhDrAsHjNWEdpVVXmgUr3XaRnkJi4aiJl71Wd4xED5BKkUT2-Axec-MIZAv9nSXm53nTrfXnRMGeMFoc5kbiMVPjhcswbYLp497d~L0FZNbQlQ0iDK-fKihzuuNhctCztRFFghvyAIOfjcVtBZV5abQZQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PP1 dose-dependently inhibits alboaggregin-A–induced tyrosine phosphorylation of FcR γ-chain, Syk, and PLCγ2.Platelets were preincubated for 3 minutes at 37°C with 0.25% dimethyl sulfoxide (DMSO) or various concentrations of PP1 before stimulation with alboaggregin-A (3.5 μg/mL) for 1 minute. Platelet suspensions were then lysed and either Syk or PLCγ2 immunoprecipitated. Precipitated proteins were then separated by SDS-PAGE and immunoblotted with 4G10 [A(i) and B(i)] and subsequently with either anti-Syk [A(ii)] or anti-PLCγ2 [B(ii)]. Immunoblots shown are representative of 3 separate experiments.
