CD36 peptides inhibit the binding of¹²⁵I-PfEMP1 to CD36. TX100 insoluble material from surface iodinated PE was extracted with SDS (SDS extract) and reconstituted in RPMI containing 1% BSA and 0.5% TX100.35 Peptides dissolved in DMSO were added to the sample, incubated overnight, and allowed to bind to CD36-coated beads for 3 hours. The beads were washed 3 times and processed for SDS-PAGE as before.35 Peptides were tested at 500 μmol/L, except for peptide CD36 233-255, which was tested at 300 μmol/L. All samples except No DMSO, had a 5% final concentration of DMSO. The percent inhibition of binding from the DMSO control is indicated at the bottom of the figure. Negative numbers means higher binding than the DMSO control. (A) MC R⁺ SDS extract; (B) FCR₃-C5 SDS extract. The percent inhibition of peptide CD36 358-370 with FCR3-C5 extract was calculated without the specific background subtraction.